Journal: Current Biology

Article Title: A receptor required for chitin perception facilitates arbuscular mycorrhizal associations and distinguishes root symbiosis from immunity

doi: 10.1016/j.cub.2024.03.015

Figure Lengend Snippet:

Article Snippet: X-Gluc , Goldbio , B735.

Techniques: Recombinant, Protease Inhibitor, Agarose Gel Electrophoresis, Software

Journal: International Journal of Molecular Sciences

Article Title: The Identification of Novel Anti-Inflammatory Effects of Cannabigerol in the Kidney Tissue of Rats Subjected to a High-Fat High-Sucrose Diet

doi: 10.3390/ijms26073114

Figure Lengend Snippet: The expression of extracellular matrix proteins, i.e., matrix metalloproteinase 2 (MMP−2; ( A )), matrix metalloproteinase 9 (MMP−9; ( B )), collagen type 1 α 1 (COL−1α1; ( C )), and collagen type 3 α 1 (COL−3α1; ( D )) regulating fibrotic processes in the kidney tissue of rats subjected to a standard diet (Control) or high-fat high-sucrose diet (HFHS) after two weeks of supplementation of cannabigerol (CBG) solution. The results are shown as the mean ± standard deviation (SD) and based on six individual measurements in each experimental group. * p < 0.05—significant differences.

Article Snippet: The Western blot technique was applied to assess the expression of proteins involved in the synthesis pathways of eicosanoid, specifically cyclooxygenase-1 (COX-1; Santa Cruz Biotechnology, Inc., Dallas, TX, USA), cyclooxygenase-2 (COX-2; Santa Cruz Biotechnology, Inc., Dallas, TX, USA), 5-lipoxygenase (5-LOX; Abcam, Cambridge, UK), and 12/15-lipoxygenase (12/15-LOX; Santa Cruz Biotechnology, Inc., Dallas, TX, USA); the expression of other proteins regulated inflammatory state development, specifically nuclear factor erythroid 2-related factor 2 (Nrf-2; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) and nuclear factor κ B (NF-κB; Cell Signaling Technology, Inc., Danvers, MA, USA); and the expression of proteins regulated the extracellular matrix (ECM) composition, specifically matrix metalloproteinase 2 (MMP-2; Santa Cruz Biotechnology, Inc., Dallas, TX, USA), matrix metalloproteinase 9 (MMP-9; Santa Cruz Biotechnology, Inc., Dallas, TX, USA), collagen type 1 α 1 (COL-1α1; Santa Cruz Biotechnology, Inc., Dallas, TX, USA), and collagen type 3 α 1 (COL-3α1; Santa Cruz Biotechnology, Inc., Dallas, TX, USA).

Techniques: Expressing, Control, Standard Deviation